Generator

Part:BBa_K638403:Design

Designed by: Joseph Harvey   Group: iGEM11_Cambridge   (2011-09-20)


Arabinose inducible TorA-Reflectin A1-sfGFP generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1369
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2437


Design Notes

NA


Source

This construct was assembled from its parts using Gibson Assembly. The reflectin A1 sequence was kindly provided by Dr Wendy Crookes-Goodson. The TorA tag was synthesised in two overlapping pieces, which were combined by PCR.

References